Technical

Extracel™ Trial Kit (5.0 mL) Product Data Sheet

For research use only

PRODUCT DESCRIPTION

The Extracel™ Trial Kit is composed of Glycosil™ (CMHA-S, thiol-modified hyaluronic acid), Gelin-S™ (GTN-DTPH, thiol-modified gelatin), Extralink™ (PEGDA, polyethylene glycol diacrylate), and degassed, deionized water (DG Water™).

Solutions of Glycosil™ and Gelin-S™ form a transparent hydrogel when mixed with Extralink™. All lyophilized solids are blanketed by argon and under a slight vacuum.

STORAGE

Glycosil™and Gelin-S™

  • Store Glycosil™ and Gelin-S™ in original vials at -20 °C for up to one year.
  • Solutions can be stored at -20 °C for ~ one month if they are frozen immediately after reconstitution in their original argon blanketed vials.
  • Do not mix Glycosil™ and Gelin-S™ prior to freezing.
  • Freeze/thaw solutions a minimum number of times for best results.
  • Solutions should be thawed at 37 °C on a rocker and used immediately after thawing.
  • During thawing, solutions go through a gel-like phase before returning to their liquid state.
  • Do not uncap the Glycosil™ and Gelin-S™ vials, since both materials disulfide crosslink in the presence of oxygen.
  • Use a syringe to add DG Water™ and remove product from the vials.

Extralink™

  • Store Extralink™ in the original vial at -20 °C for up to one year.
  • Reconstituted solutions can be stored at -20°C for about a month.

INSTRUCTIONS FOR USE

Glycosil™, Gelin-S™, and Extralink™ solutions are prepared by dissolving the lyophilized solids in the DG Water™. When reconstituted, they will be in phosphate buffered saline (PBS) buffer pH ~7.4.

Extracel hydrogels (5.0 mL) should be prepared in the following manner:

  1. Remove Glycosil™, Gelin-S™, and Extralink™ vials from the -20 °C freezer and heat them to 37 °C (~30 minutes).
  2. Remove the DG Water™ from the -20 °C freezer and thaw in a 37 °C water bath (~15 minutes).
  3. Under aseptic conditions and using a syringe with the exact amount of liquid, add 2.0 mL of DG Water™ to the Glycosil™ vial. Repeat for the Gelin-S™ vial.
  4. Place both vials horizontally on a rocker at 37 °C (for maximum mixing). It will take <30 minutes for the solids to fully dissolve. Solutions will be clear and slightly viscous. Note: Vigorous shaking will speed up dissolving time.
  5. Under aseptic conditions and using a syringe with the exact amount of liquid, add 1.0 mL of DG Water™ to the Extralink™ vial. Invert several times to dissolve.
  6. As soon as possible, but within four hours of making the solutions, mix equal volumes of Glycosil™ and Gelin-S™. To mix, pipette back and forth or invert the vial.
  7. If encapsulating cells, add 400 µL of cells to 4.0 mL of Glycosil™ + Gelin-S™. Pipette back and forth to mix.
  8. To form the hydrogel, add Extralink™ to the Glycosil™ + Gelin-S™ mix in a 1:4 volume ratio (1.0 mL Extralink™ to 4.0 mL Glycosil™ + Gelin-S™). The total hydrogel volume with cells is 5.4 mL.
  9. elation will occur within ~20 minutes. Note: Each kit component has been tested and shows no evidence of bacteria growth.