Technical

HyStem™-HP Vial Product Data Sheet

For research use only

PRODUCT DESCRIPTION

HyStem-HP (thiol-modified sodium hyaluronate with thiol-modified heparin) is packaged in 1 mL and 5 mL vials. Vials are blanketed by argon and under a slight vacuum.

STORAGE

Heprasil

  • Store HyStem-HP in the original vial unopened at -20 °C for up to one year.
  • Do not uncap the HyStem-HP vials since they will crosslink in the presence of oxygen. Use a syringe and needle to add DG Water to the vials.

INSTRUCTIONS FOR USE

HyStem-HP is prepared by dissolving the lyophilized solid in the DG Water. When reconstituted, it will be in 1x phosphate buffered saline (PBS), pH ~7.4. The amount of DG Water used for dissolution depends on the vial.

HyStem-HP should be prepared in the following manner:

  1. Allow the HyStem-HP vial to come to room temperature.
  2. Under aseptic conditions, using a syringe and needle, add to the vial the amount of DG Water indicated on the label.
  3. Place the vial horizontally on a rocker or shaker. It will take <30 minutes for the solids to fully dissolve. Warming to 40 °C or less and/or gently vortexing will speed up dissolving time. Solutions will be clear and slightly viscous.
  4. Typically, Extralink is used in a 1:4 volume ratio with HyStem-HP (i.e. 1.0 mL of HyStem-HP is crosslinked with 0.25 mL of Extralink). Note: Hydrogels made using only HyStem-HP and Extralink will not support cell attachment. Use with Gelin-S for cell attachment.

Note: Product has been manufactured under aseptic conditions and tested for bacteria and fungus.

For more information about HyStem-HP, please go to www.glycosan.com.