Technical

HyStem™-HP Hydrogel Kit (7.5 mL) Product Data Sheet

For research use only

PRODUCT DESCRIPTION

The HyStem™-HP Hydrogel Kit is composed of HyStem™-HP (thiol-modified sodium hyaluronate with thiol-modified heparin), Gelin-S™ (thiol modified gelatin), Extralink™ (PEGDA, polyethylene glycol diacrylate), and degassed, deionized water (DG Water). Solutions of HyStem-HP and Gelin-S form a transparent hydrogel when mixed with Extralink. All lyophilized solids are blanketed by argon and under a slight vacuum.

STORAGE

HyStem-HP and Gelin-S

  • Store HyStem-HP and Gelin-S in original vials unopened at -20 °C for up to one year.
  • Do not uncap the HyStem-HP and Gelin-S vials since both materials will crosslink in the presence of oxygen. Use a syringe and needle to add DG Water.

Extralink™

  • Store Extralink in the original vial unopened at -20 °C for up to one year.
  • Reconstituted Extralink solutions can be stored at -20 °C for ~ one month.

INSTRUCTIONS FOR USE

HyStem-HP, Gelin-S and Extralink solutions are prepared by dissolving the lyophilized solids in the DG Water. When reconstituted, the three materials will be in 1x phosphate buffered saline (PBS), pH ~7.4.

HyStem-HP hydrogels (3 x 2.5 ml = 7.5 mL) should be prepared in the following manner:

  1. Allow the HyStem-HP, Gelin-S, Extralink, and DG Water vials to come to room temperature.
  2. Under aseptic conditions, using a syringe and needle, add 1.0 mL of DG Water to the HyStem-HP vial. Repeat for the Gelin-S vial..
  3. Place both vials horizontally on a rocker or shaker. It will take <30 minutes for the solids to fully dissolve. Warming to not more than 37 °C and/or gently vortexing will speed dissolution,   Solutions will be clear and slightly viscous.
  4. Under aseptic conditions, using a syringe and needle, add 0.5 mL of DG Water to the Extralink vial. Invert several times to dissolve.
  5. As soon as possible, but within 2 hours of making the solutions, aseptically mix equal volumes of HyStem-HP and Gelin-S™. To mix, pipette back and forth slowly to avoid trapping air bubbles.
  6. If adding growth factors, add the appropriate amount of sterile growth factor to the HyStem-HP + Gelin-S solution and pipette back and forth to mix.
  7. If encapsulating cells, resuspend cell pellet in 2.0 mL of HyStem-HP + Gelin-S. Pipette back and forth to mix.
  8. To form the hydrogel, add Extralink to the HyStem-HP + Gelin-S mix in a 1:4 volume ratio (0.5 mL Extralink™ to 2.0 mL HyStem-HP+ Gelin-S) and mix by pipette.
  9. Gelation will occur within ~20 minutes.

Note: Each kit component has been manufactured under aseptic conditions and tested for bacteria and fungus.

For more information about our HyStem Hydrogel Kits, please go to www.glycosan.com.