Technical

Cell Types Cultivated in Extracel™ Technology

For research use only

Primary Cells

  • Human primary hepatocytes1,2
  • Rat primary hepatocytes1,2
  • Human tracheal scar fibroblasts3,4
  • Human dermal fibroblasts5,6
  • Human fetal osteoblasts7
  • Chick dorsal root ganglia8

Stem Cells

  • Human embryonic stem cells (H9)9
  • Human mesenchymal stem cells9,10
  • Rabbit mesenchymal stem cells11
  • CD34+ stem cells derived from human-cord blood9
  • Hepatic stem cells12
  • Hepatoblasts12

Cell Lines

  • HepG2 C3A cells1,2
  • HepG2 cells1,2
  • MDCK cells2,13
  • L929 fibroblasts14
  • NIH 3T3 fibroblasts15
  • MCF-10A2

Cancer-Cell Lines

  • A549 (lung)2
  • MDA-MB-468 (breast)17
  • MDA-MB-231 (breast)17
  • MCF-7 (breast)17
  • OVCAR-3 (ovarian)17
  • SK-OV-3 (ovarian)17
  • HCT-116 (colon)2
  • Caco-2 (colon)17
  • MiaPaCa-2 (pancreatic)16

References

  1. G. D. Prestwich, Y. Liu, M. Serban, B. Yu, X. Z. Shu, and A. Scott, “3-D Culture in Synthetic Extracellular Matrices: New Tissue Models for Drug Toxicology and Cancer Drug Discovery,” invited, Adv. Enz. Res., in press (2007).
  2. Unpublished data from G. D. Prestwich, et al, University of Utah.
  3. Y. Liu, Z. X. Shu, S. D. Gray, G. D. Prestwich, “Disulfide-crosslinked Hyaluronan-Gelatin Sponge: Growth of Fibrous Tissue In Vivo,” J Biomed Mat Res, 68A, 142-149 (2004).
  4. X. Z. Shu, Y. Liu, F. Palumbo, Y. Luo, G. D. Prestwich, “In Situ Crosslinkable Hyaluronan Hydrogels for Tissue Engineering,” Biomaterials, 25, 1339-1348 (2004).
  5. K. Ghosh, Z. Pan, E. Guan, S. Ge, Y. Liu, T. Nakamura, X. Ren, M. Rafailovich, R. Clark, “Cell Adaptation to a Physiologically Relevant ECM Mimic with Different Viscoelastic Properties,” Biomaterials 28, 671-679 (2007).
  6. G. D. Prestwich, X. Z. Shu, Y. Liu, S. Cai, J. F. Walsh, C. W. Hughes, K. R. Kirker, R. R. Orlandi, A. H. Park, S. L. Thibeault, M. E. Smith, “Injectable Synthetic Extracellular Matrices for Tissue Engineering and Repair,” Adv. Exp. Med. Biol., 585, 125-133 (2006).
  7. Unpublished data from Dietmar Hutmacher, et al, University of Singapore.
  8. E. M. Horn, M. Beaumont, X. Z. Shu, A. Harvey, G. D. Prestwich, K. M. Horn, A. R. Gibson, M. C. Preul, A. Panitch, “Influence of cross-linker hyaluronic acids on neurite outgrowth and recovery from spinal cord injury,” J of Neurosurg Spine, 6(2), 133-40 (2007).
  9. Unpublished data from Linda Kelley, et al, University of Utah
  10. Unpublished data from Yongzhi Qiu, Robert McCall, Vladimir Mironov, Xuejun Wen, Clemson University, and Medical University of South Carolina.
  11. Y. Liu, X. Z. Shu, G. D. Prestwich, “Osteochondral defect repair with autologous bone marrow derived MSC cells in an injectable in situ crosslinked synthetic extracellular matrix,” Tissue Engineering, 12(12), 3405-3416 (2006).
  12. Lola Reid lab, submitted data, University of North Carolina
  13. Unpublished data from T. Tandeski, G. D. Prestwich, L. Kelley, University of Utah.
  14. Y. Liu, X. Z. Shu, G. D. Prestwich, “Biocompatibility and Stability of Disulfide-Crosslinked Hyaluronan Films,” Biomaterials, 26, 4737-4746 (2005).
  15. X. Z. Shu, Y. Liu, Y. Luo, M. C. Roberts, and G. D. Prestwich, “Disulfide-Crosslinked Hyaluronan Hydrogels,” Biomacromolecules, 3, 1304-1311 (2002).
  16. Unpublished data from C. Scaife, et al, University of Utah.
  17. Y. Liu, X. Z. Shu, and G. D. Prestwich, “Tumor Engineering: Orthotopic Cancer Models in Mice Using Cell-Loaded, Injectable, Crosslinked Hyaluronan-Derived Hydrogels,” Tissue Engineering 13(5), 1091-1101(2007).