Extracel™ Sponge

Extracel™ Sponge-96

The Extracel Sponge-96 offers the advantages of Glycosan’s Extracel formula in a convenient, lyophilized sponge that retains its porous structure when rehydrated. It is ideal for culturing primary cells and cell lines in 3-D or tissue engineering applications requiring a defined pore structure for efficient gas exchange and fluid transfer1,2,3. Unlike animal-derived extracellular matrices (ECM), Extracel Sponge-96 is chemically fully defined. The sponges are based on three biocompatible components: thiol-modified hyaluronan (a major constituent of native ECM), thiol-modified gelatin (denatured collagen), and a thiol-reactive crosslinker (polyethylene glycol diacrylate).

Format

  • The Extracel Sponge-96 comes in a 96-well plate pre-filled with ready to use sponges that have a volume of 100 µl.

Applications

3-D cell culture

The Extracel Sponge-96 provides a basic scaffold for 3-D cell growth that closely matches the extracellular environment. It is suitable for a broad range of cell culture applications, including the cultivation of hepatocytes and primary human tracheal scar fibroblasts4.

References

  1. Y. Liu, Z.X. Shu, S.D. Gray, G.D. Prestwich, “Disulfide-crosslinked Hyaluronan-Gelatin Sponge: Growth of Fibrous Tissue In Vivo,” J Biomed Mat Res, 68A, 142-149 (2004).
  2. Y. Liu, S. Ahmad, X.Z. Shu, R.K. Sanders, S.A. Kopesec, G.D. Prestwich, “Accelerated repair of cortical bone defects using a synthetic extracellular matrix to deliver human demineralized bone matrix, J Orthop Res, 24(7), 1454-1462 (2006).
  3. Y. Liu, X.Z. Shu, and G.D. Prestwich, “Osteochondral Defect Repair with Autologous Bone Marrow-Derived Mesenchymal Stem Cells in an Injectable, in situ Crosslinked Synthetic Extracellular Matrix,” Tissue Eng., 12, 3405-3416 (2006).
  4. M.A. Serban, Y. Liu, and G.D. Prestwich, “Effects of Synthetic Extracellular Matrices on Primary Human Fibroblast Behavior,” Acta Biomaterialia, 4, 67-75 (2008).