Vitronectin
- A monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types.
- Has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.
General
Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.
Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.
Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.
Vitronectin is a glycoprotein present in plasma and tissues. When circulating in the blood, Vitronectin is found as a mixture of 75 kD and 65 kD polypeptides. Vitronectin is used to promote cell attachment, adhesion, spreading, proliferation, migration and differentiation in a variety of normal and neoplastic cells. In addition to its cell spreading function, Vitronectin binds to heparin and collagen.
Vitronectin is provided at a concentration of 0.5 mg/ml with 0.1 mg of Vitronectin being dissolved in 0.2 ml of 0.15M NaCl, 0.005M HEPES buffer at approximately pH 7.4. Vitronectin is purified from human plasma by the method of Hayman et al (1) using an anti-Vitronectin monoclonal antibody affinity column and sterilized by 0.2μ filtration.
Application
Vitronectin is used as a thin coating. The optimal concentration for cell attachment and culture may differ for various cell types. Some experimentation may be required to determine the optimal conditions for individual cell culture systems.
Vitronectin is not for human use as supplied.
Composition
Vitronectin Characteristics
|
Parameter, Testing, and Method |
Fibronectin |
|
Quantity |
0.1 mg |
|
Volume |
0.2 mL |
|
Concentration |
0.5 mg/mL |
|
Purity |
>95% |
|
Formulation |
0.15 M NaCl, 0.005M HEPES at pH 7.4 |
|
Form |
Solution |
|
Source |
Human, plasma |
|
Storage Temperature |
-20 °C |
|
Shelf Life |
12 months after receipt |
|
Sterilization Method |
Filtration |
|
Cell Attachment Activity |
Passes |
|
Sterility |
No growth |
|
Safety |
Source material found negative for infectious agents |
Vitronectin is not for human use as supplied.
Data Sheets
PRODUCT DESCRIPTION
Vitronectin is a glycoprotein present in plasma and tissues. When circulating in the blood, Vitronectin is found as a mixture of 75 kD and 65 kD polypeptides. Vitronectin is used to promote cell attachment, adhesion, spreading, proliferation, migration and differentiation in a variety of normal and neoplastic cells. In addition to its cell spreading function, Vitronectin binds to heparin and collagen.
Vitronectin is provided at a concentration of 0.5 mg/ml with 0.1 mg of Vitronectin being dissolved in 0.2 ml of 0.15M NaCl, 0.005M HEPES buffer at approximately pH 7.4. Vitronectin is purified from human plasma by the method of Hayman et al (1) using an anti-Vitronectin monoclonal antibody affinity column and sterilized by 0.2μ filtration.
STORAGE
It is recommended that Vitronectin be stored below -20°C and repeat thawing and freezing be avoided.
CHARACTERIZATION
Source: Human plasma
- Purity: Vitronectin has a purity of >95% based on Coomassie brilliant blue stain of 7.5% SDSPAGE. Fibronectin contamination is less than 0.04% based on immunoblotting.
- Concentration: The concentration of Vitronectin is 0.5 mg/ml with 0.1 mg of Vitronectin being dissolved in 0.2 ml of buffer at approximately pH 7.4.
- Cell Attachment Activity: 24 well plates were coated with Vitronectin in PBS (0.3 ml/well) at 37°C overnight. After blocking by BSA, TIG-3 cells (JCRB 0506) (5 X 104) cells/ 1 ml DMEM/well) were added and incubated at 37°C for 90 minutes. Attached cells were counted with a Coulter counter.
- pH: Vitronectin is dissolved in buffer with the pH being approximately 7.4.
INSTRUCTIONS FOR USE
Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Thaw Vitronectin and dilute to desired concentration using serum-free medium or PBS. The final solution should be sufficiently diluted so that the volume added covers the surface evenly.
- Add appropriate amount of diluted material to culture surface.
- Incubate at room temperature for 1-2 hours.
- Aspirate remaining material.
- Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
- Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
PRECAUTIONS
The human-source raw material used in the production of this product tested negative for hepatitis B virus, hepatitis C virus (HCV), human immunodeficiency virus type-1 (HIV-1) and type-2 (HIV-2) and Treponema pallidum. Handle as if potentially infectious.
