- Thiol-reactive crosslinker (polyethylene glycol diacrylate – PEGDA)
- Molecular weight of 3400
- Acrylation > 95%
- High purity
- cGMP version available
BioTime now offers the highest quality Polyethylene Glycol Diacrylate (PEGDA) on the market based on head-to-head analysis with other sources of PEGDA for percent acrylation (> 95%) and purity. A cGMP-grade version is also available for those researchers who need it for their translational research and development.
Polyethylene (glycol) diacrylate-based hydrogels are powerful tools for uncovering basic cellular biology because they are considered biologically inert (“blank slate”) and their mechanical properties can be varied over a large range of moduli. In addition, PEGDA hydrogels is an emerging scaffold for tissue engineering and Regenerative Medicine since polymerization can occur rapidly at room temperature and requires low energy input, has high water content, is elastic, and can be customized to include a variety of biological molecules.
Polyethylene (glycol) diacrylate (PEGDA) is a synthetic, hydrophilic starting material which forms hydrogels in the presence of photoinitiator and UV light. PEGDA hydrogels are easily customizable since ECM proteins and/or growth factors can be incorporated into a hydrogel and its stiffness can be modulated from 10-100 kPa (9, 10). PEGDA is widely recognized as a biocompatible, non-immunogenic, and capable of chemical manipulation to incorporate attachment peptides (1, 6), degradable peptides (1,2), and other moieties (3).
- Regular and3-D cell culture (2, 3, 4, 6)
- Tissue engineering (7)
- Photolithography (5, 7, 8, 11)
For research use only
PEGDA(poly (ethylene glycol) diacrylate, Mw=3400) is packaged 100mg aliquots. Vials are blanketed by argon and under a slight vacuum.
- Store PEGDA in the original vial unopened at -20 °C for up to one year.
- Reconstituted PEGDA solutions can be stored at -20 °C for ~ one month.
INSTRUCTIONS FOR USE
PEGDA is prepared by dissolving the lyophilized solid in the DG Water; 1.0mL of DG water yields a 10w/v% solution. Typically, PEGDA is used with PEGcure Photoinitiator as a curing agent.
PEGDA hydrogels (1 mL) should be prepared in the following manner:
- Allow the PEGDA, PEGcure and DG Water to come to room temperature.
- Under aseptic conditions add 1.0 mL of DG Water to the PEGDA vial for a 10% (w/v) solution.
- Invert several times to dissolve. The solution will be clear and slightly viscous.
- Under aseptic conditions remove the 10% PEGDA solution from the vial and add entirety to the PEGcure vial. Place the vial on a rocker or shaker and allow solids to completely dissolve (approximately 10-20 minutes). The resulting solution will be 10% (w/v) PEGDA and 0.1% (w/v) PEGcure.
- Keep solution protected from light until ready to crosslink. Pipette solution into desired format (i.e. 96 well plate) and expose to UV light (wavelength 365 nm) for 15 minutes to form gels.
- Hydrogel properties may vary depending on time of exposure and type of UV light. Ensure the UV light is in close proximity to the hydrogel solution.
- Freeze unused PEGDA solution at -20 °C and protected from light.
Note: When altering this protocol gelation time and hydrogel stiffness vary depending upon the concentration of PEGDA, the concentration of PEGcure, and the duration of time exposed to UV radiation.
Note: Hydrogels made using only PEGDA and PEGcure will not support cell attachment.
Note: Product has been manufactured under aseptic conditions and tested for bacteria and fungus.
- Salinas CN and Anseth KS, The enhancement of chondrogenic differentiation of human mesenchymal stem cells by enzymatically regulated RGD functionalities. Biomaterials. (2008) 29:2370-7.
- Kloxin AM, et al.Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties Science (2009) 324, 59.
- DeForest CA, Polizzotti BD, and Anseth KS, Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments Nat. Mater. (2009) 8: pp. 659-664.
- Baird IS, Yau AY, and Mann BK, Mammalian cell-seeded hydrogel microarrays printed via dip-pin technology BioTech. (2008) 44:249-256.
- Baek TJ et al, Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-based Microreactors and Spatially Addressed Microarrays J. Microbiol. Biotechnol. (2007) 17: 1826-1832.
- Taite LJ, Rowland ML, Ruffino KA, Smith BR, Lawrence MB, West JL. Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies. Ann Biomed Eng. (2006) 34:1705-11.
- Du Y, Lo E, Ali S, Khademhosseini A. Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs. Proc Natl Acad Sci U S A. (2008) 105:9522-7.
- Khademhosseini A, Yeh J, Jon S, Eng G, Suh KY, Burdick JA, Langer R. Molded polyethylene glycol microstructures for capturing cells within microfluidic channels. Lab Chip. (2004) 4:425-30.
- Liao H, Munoz-Pinto D, Qu X, Hou Y, Grunlan MA, Hahn MS. Influence of hydrogel mechanical properties and mesh size on vocal fold fibroblast extracellular matrix production and phenotype. Acta Biomater. (2008) 4:1161-71.
- Patel PN, Smith CK, Patrick CW Jr. Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue.J Biomed Mater Res A. (2005) 73:313-9.
- Panda P et al, Stop-Flow Lithography to Generate Cell-Laden Microgel Particles. Lab Chip (2008) 8:1056-1061.
- Ma PX and Elisseeff J editors, Scaffolds in Tissue Engineering, CRC Press Boca Raton, FL 2006.