Extracel-X™ Hydrogel Kit
Extracel-X™ is recommended for growing tumor xenografts since it has been shown to support growth of a wide variety of tumor cancer cell types (both orthotopically and subcutaneously) in mice1.
Extracel-X™ is a synthetic extracellular matrix (ECM) used to deliver and cultivate cancer cells in vivo and for tissue-engineering applications (with and without cells). The Extracel-X™ solution can be injected and crosslinked in situ. Unlike animal-derived extracellular matrices (ECM), Extracel-X™ is chemically defined and nonimmunogenic. Extracel-X™ is based on three biocompatible components: thiol-modified hyaluronan (a major constituent of native ECM), thiol-modified gelatin (denatured collagen), and a thiol-reactive crosslinker (polyethylene glycol diacrylate, PEGDA).
Gelation
Reconstituted Extracel-X™ components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent Extralink™ (PEGDA) is added to Glycosil™ (thiol-modified hyaluronan) and Gelin-S™ (thiol-modified gelatin). Gelation occurs from twenty to more than 120 minutes after all three components are mixed, depending on user requirements. No steps depend on low temperature or low pH.
3D Cell Recovery Matrix
The Extracel-X Hydrogel is also avalable with a noval crosslinker which enables the gell to be broken down for easy encapsulated cell recovery. At checkout simple select PEGSSDA as your crosslinker.