Gelin-S™

Gelin-S™

Gelin-S™ is thiol-modified gelatin (denatured collagen) and is a component of the Extracel™, Extracel-X™, Extracel-HP™, Extracel-HPG™, and Extracel-LG™ hydrogel kits. Most cells do not grow well on Gelin-S™–only hydrogels1. Instead, Gelin-S™ should be used in conjunction with Glycosil™ (thiol-modified hyaluronic acid) or Heprasil™ (thiol-modified hyaluronic acid with thiol-modified heparin). Gelin-S™ can be purchased separately from the hydrogel kits in individual vials.

Gelation

Reconstituted Gelin-S™ remains liquid at 15 to 37°C. One cannot make a Gelin-S™–only hydrogel because it will not gel either with or without the Extralink™ crosslinker.

Volume

Gelin-S™ vials come in two sizes:

  1. 1 mL
  2. 5 mL

Gelatin Source

The gelatin used to make Gelin-S™ is Type B Gelatin, Bloom 225, derived from bovine skin:
(http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/G9391).

Gelatin is a heterogeneous mixture of water-soluble collagens with high, average molecular weights. The proteins are extracted by boiling lime-cured bovine skin in water. Glycosan is currently investigating the use of porcine-derived gelatin in place of this material.

References

  1. X. Z. Shu, S. Ahmad, Y. Liu, and G. D. Prestwich, “Synthesis and Evaluation of Injectable, In Situ Crosslinkable Synthetic Extracellular Matrices (sECMs) for Tissue Engineering,” J. Biomed Mater. Res. A, 79A(4), 901-912 (2006).