Vitronectin

Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.

Vitronectin is a glycoprotein present in plasma and tissues. When circulating in the blood, Vitronectin is found as a mixture of 75 kD and 65 kD polypeptides. Vitronectin is used to promote cell attachment, adhesion, spreading, proliferation, migration and differentiation in a variety of normal and neoplastic cells. In addition to its cell spreading function, Vitronectin binds to heparin and collagen.

Vitronectin is provided at a concentration of 0.5 mg/ml with 0.1 mg of Vitronectin being dissolved in 0.2 ml of 0.15M NaCl, 0.005M HEPES buffer at approximately pH 7.4. Vitronectin is purified from human plasma by the method of Hayman et al (1) using an anti-Vitronectin monoclonal antibody affinity column and sterilized by 0.2μ filtration.